Rigid gigaporous chromatographic media and their potential impact on downstream processing--颗粒学报PARTICUOLOGY

Gu, T., Zhou, W., Ma, G., & Su, Z. (2005). Rigid gigaporous chromatographic media and their potential impact on downstream processing. China Particuology, 3(6), 349-353. https://doi.org/10.1016/S1672-2515(07)60214-0

Rigid gigaporous chromatographic media and their potential impact on downstream processing

Tingyue Gu ^a *, Weiqing Zhou ^b, Guanghui Ma ^b, Zhiguo Su ^b

a Department of Chemical Engineering, Ohio University, Athens, Ohio 45701, USA
b Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, P. R. China

10.1016/S1672-2515(07)60214-0

Volume 3, Issue 6, December 2005, Pages 349-353

Received 29 September 2005, Accepted 18 November 2005, Available online 27 November 2007.

E-mail: gu@ohio.edu

Highlights

Abstract

More and more biomolecules are being produced by the biotechnology industry for applications ranging from medicine and food to engineering materials. Liquid chromatography plays a center-stage role in a typical downstream process producing biomolecules such as recombinant proteins. Rigid gigaporous media are porous particles possessing large transecting through-pores with a pore-to-particle diameter ratio of dpore/dparticle> 0.01. They allow convective flow in the large through-pores, while the smaller diffusion-pores (typically several hundred angstroms in size) supply the needed surface areas. Because of the transecting gigapores, a portion of the mobile phase flows through the pores in addition to fluid flow in the interstitial spaces between the particles in a packed-bed column. This considerably lowers the operating column pressure drop. This lower pressure drop makes axial-direction scale-up of chromatographic columns possible to avoid pancake columns that invariably degrade separation resolution. The large gigapores also make the binding sites on the diffusion pore surfaces more accessible, thus increasing the loading capacity of large protein molecules that can be hindered sterically if only diffusion pores are present. This work discusses the development of rigid gigaporous media and their potential impact on the design of multi-stage downstream process from the angle of multi-scale analysis.

Graphical abstract

Keywords

Gigapore; chromatography; bioseparation; downstream process; multi-scale; diffusion

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