- Volumes 84-95 (2024)
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Volumes 72-83 (2023)
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Volume 83
Pages 1-258 (December 2023)
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Volume 82
Pages 1-204 (November 2023)
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Volume 81
Pages 1-188 (October 2023)
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Volume 80
Pages 1-202 (September 2023)
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Volume 79
Pages 1-172 (August 2023)
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Volume 78
Pages 1-146 (July 2023)
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Volume 77
Pages 1-152 (June 2023)
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Volume 76
Pages 1-176 (May 2023)
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Volume 75
Pages 1-228 (April 2023)
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Volume 74
Pages 1-200 (March 2023)
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Volume 73
Pages 1-138 (February 2023)
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Volume 72
Pages 1-144 (January 2023)
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Volume 83
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Volumes 60-71 (2022)
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Volume 71
Pages 1-108 (December 2022)
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Volume 70
Pages 1-106 (November 2022)
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Volume 69
Pages 1-122 (October 2022)
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Volume 68
Pages 1-124 (September 2022)
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Volume 67
Pages 1-102 (August 2022)
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Volume 66
Pages 1-112 (July 2022)
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Volume 65
Pages 1-138 (June 2022)
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Volume 64
Pages 1-186 (May 2022)
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Volume 63
Pages 1-124 (April 2022)
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Volume 62
Pages 1-104 (March 2022)
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Volume 61
Pages 1-120 (February 2022)
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Volume 60
Pages 1-124 (January 2022)
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Volume 71
- Volumes 54-59 (2021)
- Volumes 48-53 (2020)
- Volumes 42-47 (2019)
- Volumes 36-41 (2018)
- Volumes 30-35 (2017)
- Volumes 24-29 (2016)
- Volumes 18-23 (2015)
- Volumes 12-17 (2014)
- Volume 11 (2013)
- Volume 10 (2012)
- Volume 9 (2011)
- Volume 8 (2010)
- Volume 7 (2009)
- Volume 6 (2008)
- Volume 5 (2007)
- Volume 4 (2006)
- Volume 3 (2005)
- Volume 2 (2004)
- Volume 1 (2003)
Protein expression in E. coli often results in the formation of a kind of protein aggregate called inclusion body. Conversion of the inactive protein aggregate into biologically active protein is a key step in production of recombinant products. Conventional dilution refolding technique suffers from disadvantages of low recovery and low concentration. Various chromatographic refolding techniques have been developed over the last few years. These include size-exclusion chromatography, ion exchange chromatography, hydrophobic interaction chromatography and different affinity chromatography. A successful strategy is the use of gradient elution in column control which provides a gentle and gradual change of the solution environment for the macromolecule to refold at nano-scale. The gradient refolding at column scale could minimize misfolding and aggregation which are induced by sudden change of the solution in conventional refolding operation.