Enabling multienzyme bioactive systems using a multiscale approach--颗粒学报PARTICUOLOGY

Wang, P., Ma, G., Gao, F., & Liao, L. (2005). Enabling multienzyme bioactive systems using a multiscale approach. China Particuology, 3(6), 304-309. https://doi.org/10.1016/S1672-2515(07)60207-3

Enabling multienzyme bioactive systems using a multiscale approach

Ping Wang ^{a b *}, Guanghui Ma ^b, Fei Gao ^b, Liang Liao ^a

a Department of Chemical and Biomolecular Engineering, The University of Akron, Akron, OH 44325, USA
b National Key Lab of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, P. R. China

10.1016/S1672-2515(07)60207-3

Volume 3, Issue 6, December 2005, Pages 304-309

Received 10 November 2005, Accepted 26 November 2005, Available online 27 November 2007.

E-mail: wangp@uakron.edu

Highlights

Abstract

The potentials of multi-scale design that combines both nanoscale and microscale mechanisms for biocatalysis involving multiple enzymes and cofactor(s) are examined. Performance of these complex systems depends on proper interactions among the enzymes and cofactor(s). In this work, nanoparticle-based multiple enzymes and tethered cofactor are designed to stimulate such interactions. Furthermore, the nanostructures are encapsulated in microcapsules with membranes possessing pores that are comparable to the size of nanoparticles. While the nanoparticles are the “motor” driving the complex reactions inside the capsules, the nano-sized pores of the microspheres allow efficient molecular diffusion for rapid reactant supply and product removal. The microcapsules can then be used in form of packed bed or suspension reactors for large-scale industrial operations. The multi-level design provides the mechanism for matching reaction and mass transfer rates, and for optimizing the volumetric productivity. This study showcases an interesting concept which entails interactions among nanostructured multiple enzymes and cofactor, the integration of complex nano-scale catalytic structures into micro-scale and then industrial process-scale systems for long-term continuous operations.

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