Effects of laponite and silica nanoparticles on the cleaning performance of amylase towards starch soils--颗粒学报PARTICUOLOGY

Lee, W. P., Martinez, A., Xu, D., Brooker, A., York, D. W., & Ding, Y. (2009). Effects of laponite and silica nanoparticles on the cleaning performance of amylase towards starch soils. Particuology, 7(6), 459–465. https://doi.org/10.1016/j.partic.2009.09.001

Effects of laponite and silica nanoparticles on the cleaning performance of amylase towards starch soils

Wai Peng Lee ^a, Alberto Martinez ^b, Dan Xu ^a, Anju Brooker ^b, David W. York ^b, Yulong Ding ^a *

a Institute of Particle Science & Engineering, University of Leeds, Leeds LS2 9JT, UK
b Procter & Gamble Newcastle Technical Centre, Newcastle upon Tyne NE12 9TS, UK

10.1016/j.partic.2009.09.001

Volume 7, Issue 6, December 2009, Pages 459-465

Received 1 January 2009, Revised 7 July 2009, Accepted 15 September 2009, Available online 14 October 2009.

E-mail: y.ding@leeds.ac.uk

Highlights

Abstract

This work aims to understand the effect of nanoparticle–enzyme interactions and how such interactions affect starch based soil removal. Silica and laponite are used as the model nanoparticles, and α-amylase is employed as the model enzyme. The results show that, if the nanoparticles and enzyme are added simultaneously, laponite enhances the enzyme performance toward starch soil removal, whereas silica imposes a small effect on the enzymatic activity towards the same soil substrates. However, when nanoparticles are added first, the enzyme activity is not affected much by laponite but is hindered significantly by silica nanoparticles. Furthermore, sequential addition of the enzyme followed by silica nanoparticles improves soil removal. Electron microscopic analyses, measurements of the enzyme activity in suspensions of nanoparticles, and particle size characterisation suggest that dense coverage of soil surface by the silica nanoparticles be likely a mechanism for the experimentally observed hindrance of soil removal when silica nanoparticles are added before enzyme.

Graphical abstract

Keywords

Enzyme; Nanoparticles; Laponite; Silica; Interaction; Starch

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