Depressing defocusing effect in microscopy by bi-orthogonal wavelet transform--颗粒学报PARTICUOLOGY

Sun, H., Wang, J., & Shao, R. (2012). Depressing defocusing effect in microscopy by bi-orthogonal wavelet transform. Particuology, 10(5), 644–647. https://doi.org/10.1016/j.partic.2012.02.005

Depressing defocusing effect in microscopy by bi-orthogonal wavelet transform

Hequan Sun ^a *, Jiguang Wang ^a, Ruoli Shao ^b

a Department of Military Oceanography, Dalian Naval Academy, Dalian 116018, China
b Design Institute of Civil Engineering and Architecture, Dalian University of Technology, Dalian 116023, China

10.1016/j.partic.2012.02.005

Volume 10, Issue 5, October 2012, Pages 644-647

Received 4 March 2011, Revised 29 September 2011, Accepted 20 February 2012, Available online 5 May 2012.

E-mail: hqsun@dl.cn

Highlights

► Bi-orthogonal wavelet transform was used to depress defocus effect by merging the wavelet coefficients of the image series taken by microscope.

► Satisfactory results were obtained by using wavelets CDF(2,2) and SWE(13,7).

Abstract

For different dimensions, heights and irregular shapes, small and large particles cannot be viewed simultaneously as sharp images under the microscope due to optical defocusing effects. Bi-orthogonal wavelet transform is used in this paper to depress such defocusing effect by merging the wavelet coefficients of the image series. The algorithm is presented in detail, using SWE(13,7) and CDF(2,2), and a few particle images are provided to validate the proposed method.

Graphical abstract

Keywords

Defocusing effect; Wavelet transform; Bi-orthogonal wavelet; CDF(2,2); SWE(13,7)

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