Surface charge of PLA microparticles in regulation of antigen loading, macrophage phagocytosis and activation, and immune effects in vitro--颗粒学报PARTICUOLOGY

Liu, Y., Chen, X., Wang, L., Yang, T., Yuan, Q., & Ma, G. (2014). Surface charge of PLA microparticles in regulation of antigen loading, macrophage phagocytosis and activation, and immune effects in vitro. Particuology, 17, 74–80. https://doi.org/10.1016/j.partic.2014.02.006

Surface charge of PLA microparticles in regulation of antigen loading, macrophage phagocytosis and activation, and immune effects in vitro

Yuying Liu ^{a b}, Xiaoming Chen ^{a c}, Lianyan Wang ^a *, Tingyuan Yang ^a, Qipeng Yuan ^b, Guanghui Ma ^a

a National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China
b State Key Laboratory of Chemical Resource Engineering, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
c University of Chinese Academy of Sciences, Beijing 100049, China

10.1016/j.partic.2014.02.006

Volume 17, December 2014, Pages 74-80

Received 24 July 2013, Revised 17 February 2014, Accepted 28 February 2014, Available online 3 May 2014.

E-mail: wanglianyan@home.ipe.ac.cn

Highlights

• PLA microparticles coated with various polymers were prepared, having different surface charges.

• The MPs were used as adjuvants to examine effect of surface charge on its adjuvanticity in vitro.

• HBsAg was loaded on the MPs as antigen and RAW 264.7 was used as macrophage.

• Surface charges helped to increase antigen loading, and internalization of antigen into macrophages.

• Surface charge also promoted expression of MHC II, CD80 and improved secretion level of TNF-α.

Abstract

The use of microparticles (MPs) as adjuvants has attracted increasing interest in vaccine delivery systems. Many physiochemical characteristics of MPs including hydrodynamic size, surface properties, and morphology can regulate the immune response. Surface charge is an important characteristic of MPs, but how it affects their adjuvanticity remains unknown. In this study, we prepared uniform-sized polylactide MPs coated with various polymers of different positive charge, and investigated how the surface charge affected antigen loading and macrophage phagocytosis and activation in vitro. A higher surface charge greatly enhanced antigen loading and antigen internalization into macrophages, promoted the expression of MHC II and CD80, and increased the secretion level of TNF-α. Taken together, these results indicated that surface charge was an important parameter for improving the adjuvanticity of MPs.

Graphical abstract

Keywords

Microparticle (MP); Macrophage; Surface charge; Antigen loading; Phagocytosis; Activation

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