Comparison of the effects of different protocols on the particle size distribution of TiO2 dispersions--颗粒学报PARTICUOLOGY

Tantra, R., Sikora, A., Hartmann, N. B., Sintes, J. R., & Robinson, K. N. (2015). Comparison of the effects of different protocols on the particle size distribution of TiO2 dispersions. Particuology, 19, 35–44. https://doi.org/10.1016/j.partic.2014.03.017

Comparison of the effects of different protocols on the particle size distribution of TiO₂ dispersions

Ratna Tantra ^a *, Aneta Sikora ^a, Nanna B. Hartmann ^b, Juan Riego Sintes ^b, Kenneth N. Robinson ^a

a National Physical Laboratory, Teddington, Middlesex TW11 0LW, UK
b European Commission, DG Joint Research Centre, Ispra, Italy

10.1016/j.partic.2014.03.017

Volume 19, April 2015, Pages 35-44

Received 13 January 2014, Revised 12 March 2014, Accepted 27 March 2014, Available online 22 July 2014.

E-mail: ratna.tantra@npl.co.uk

Highlights

• Variability in dispersion characteristics of TiO₂ nanopowder between protocols was assessed.

• The results highlighted the current comparability problem between many nanoecotoxicology studies.

• Concentration of nanomaterial was the largest source of variation in the protocols studied.

• Nanomaterial sampling and sonication time were also significant factors.

Abstract

There is a need to standardize methods associated with the dispersion of nanomaterials in nano(eco)toxicological investigations. The first step toward this goal is to understand the degree of variability that exists in nanomaterial dispersions prepared by using different protocols. Using two case studies, the degree of variability in TiO₂ nanomaterial dispersions was assessed by differential centrifugal sedimentation (DCS) methods. Case study 1 addresses the variability that arises from variations within one protocol, investigating the effects of dispersion aging, sonication exposure time, sonication in the presence/absence of an ice bath, material subsampling, particle concentration and having a pre-wetting step. Case study 2 addresses the variability between four different protocols developed through past research activities and projects. The results indicate that there is a large degree of variability (relative standard deviation (RSD) of mean particle diameter = 26%) in the dispersion of TiO₂ nanomaterials between the four different protocols studied and that several steps in a dispersion protocol are potential sources of variation, with final particle concentration being the most significant. The implication of this study is clear: for the purpose of data comparability, there is a need to provide the exact details of all steps involved in a dispersion protocol.

Graphical abstract

Keywords

Nanoparticle; DispersionProtocol; Differential centrifugal sedimentation; Nano(eco)toxicology

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