Gas-assisted magnetic separation for the purification of proteins in batch systems--颗粒学报PARTICUOLOGY

Liu, Z., Yang, L., Dong, T., Li, W., Sun, X., Zhu, M., . . . Liu, H. (2016). Gas-assisted magnetic separation for the purification of proteins in batch systems. Particuology, 24, 170-176. https://doi.org/10.1016/j.partic.2014.12.015

Gas-assisted magnetic separation for the purification of proteins in batch systems

Zhini Liu ^{a b}, Liangrong Yang ^b *, Tingting Dong ^b, Wensong Li ^{a b}, Xitong Sun ^b, Menghao Zhu^b, Zhengkang Duan ^a, Qingfen Liu ^b, Huizhou Liu ^{b *}

a College of Chemical Engineering, Xiangtan University, Xiangtan 411105, China
b Key Laboratory of Green Process and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China

10.1016/j.partic.2014.12.015

Volume 24, February 2016, Pages 170-176

Received 29 September 2014, Revised 20 November 2014, Accepted 4 December 2014, Available online 6 July 2015, Version of Record 21 January 2016.

E-mail: lryang@home.ipe.ac.cn; hzliu@home.ipe.ac.cn

Highlights

• Gas-assisted magnetic separation (GAMS) method for separating proteins was investigated.

• The method combined magnetic separation with flotation.

• High separation rate and good recovery rate were achieved under the optimal conditions.

• High recovery was obtained by adjusting initial solution pH in GAMS process.

• Large-scale recovery of protein-loaded magnetic particles would become possible.

Abstract

In this paper, gas-assisted magnetic separation (GAMS), a technique that combines magnetic separation with flotation, was investigated for the potential large-scale separation of proteins. The GAMS process includes adsorption of target proteins and magnetic separation to recover protein-loaded magnetic particles from the dilute biosuspension with the assistance of bubbles. Microsized ethylenediamine-functionalized poly(glycidyl methacrylate) superparamagnetic microspheres (MPMs) and bovine serum albumin (BSA) were used as a model system. The feasibility of GAMS for capturing BSA-loaded MPMs from an appropriate medium was shown. High recovery of BSA-loaded MPMs was obtained by simple adjustment of the initial solution pH without extra detergents and antifoaming agents. The GAMS conditions were consistent with the adsorption conditions, and no proteins were desorbed from the MPMs during this process. Under the optimal conditions, the separation rate and recovery percentage reached 410 mL/min and 98% in 0.61 min, respectively. Conformational changes of BSA during the GAMS process were investigated by fluorescence spectroscopy and circular dichroism spectrometry.

Graphical abstract

Keywords

Adsorption; Magnetic separation; Flotation; Recovery; BSA conformation

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