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• A test monodisperse aerosol generation system was designed and constructed.
• Effects of atomizer structure, minor flow rate, and oleic acid concentration were investigated.
• Performance of the constructed aerosol generation system was tested.
Monodisperse aerosols are essential in many applications, such as filter testing, aerosol instrument calibration, and experiments for validating models. This paper describes the design principle, construction, and performance of a monodisperse-aerosol generation system that comprises an atomizer, virtual impactor, microcontroller-based isokinetic probe, wind tunnel, and velocity measurement device. The size distribution of the produced monodisperse aerosols was determined by an optical particle counter. The effects of atomizer characteristics, the rates of minor and major flow, and solution criteria were investigated. It was found that all these parameters affect the generation of monodisperse aerosol. Finally, the expected geometric standard deviation (≤1.25) of monodisperse aerosol particles was obtained with the most suitable atomizer for 10% oleic acid in ethyl alcohol solution with 5%–15% minor flow, where the ratio between the nozzle-to-probe distance and acceleration-nozzle-exit diameter was 0.66. The constructed monodisperse-aerosol-generation system can be used for instrumental calibration and aerosol research.