Dual-function baicalin and baicalin-loaded poly(lactic-co-glycolic acid) nanoparticles: Immune activation of dendritic cells and arrest of the melanoma cell cycle at the G2/M phase--颗粒学报PARTICUOLOGY

Wang, H., Han, S., Wang, L., Yang, T., Zhang, G., Yu, L., & Zhao, Y. (2018). Dual-function baicalin and baicalin-loaded poly(lactic-co-glycolic acid) nanoparticles: Immune activation of dendritic cells and arrest of the melanoma cell cycle at the G2/M phase. Particuology, 37, 64-71. https://doi.org/10.1016/j.partic.2017.06.008

Dual-function baicalin and baicalin-loaded poly(lactic-co-glycolic acid) nanoparticles: Immune activation of dendritic cells and arrest of the melanoma cell cycle at the G2/M phase

Huimei Wang ^a, Shulan Han ^{a b}, Lianyan Wang ^b *, Tingyuan Yang ^b, Guifeng Zhang ^b, Lian Yu ^c, Yue Zhao ^d

a Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China
b National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China
c College of Pharmacy Jiamusi University, Jiamusi 154000, China
d Epithelial Systems Biology Laboratory, Systems Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892-1603, USA

10.1016/j.partic.2017.06.008

Volume 37, April 2018, Pages 64-71

Received 20 December 2016, Revised 18 June 2017, Accepted 24 June 2017, Available online 20 October 2017, Version of Record 3 February 2018.

E-mail: wanglianyan@ipe.ac.cn

Highlights

• Baicalin and baicalin-loaded PLGA nanoparticles (PLGA-B) were characterized for bioavailability.

• Particle size of PLGA-B prepared was ∼120 nm with a dispersity index of 0.103.

• PLGA-B could activate dendritic cells to upregulate expression of their surface marker molecules.

• PLGA-B could increase apoptosis of melanoma cells and induce cell-cycle arrest at the G2/M phase.

Abstract

Accumulating evidence suggests that the flavone glycoside baicalin has immunomodulatory effects and antitumor potential. However, its weak stability in solution, poor absorption, and low bioavailability limit its clinical application. To overcome these disadvantages, we developed baicalin-loaded poly(lactic-co-glycolic acid) nanoparticles (PLGA-B) of small size. Next, we evaluated the dual function of immunotherapy and chemotherapy for PLGA-B using immune-related cells and tumor cells. Results showed that PLGA-B were spherical, with a particle size ∼120 nm and narrow size distribution with an excellent polydispersity index of 0.103. In vitro experiments revealed that baicalin and PLGA-B could activate dendritic cells (DCs) to have higher expression of surface marker molecules and costimulatory molecules than those of control cells. Baicalin and PLGA-B could trigger apoptosis in melanoma (B16) cells via cell-cycle arrest at the G2/M phase. These data suggest that PLGA-B have important roles in activating DCs and killing melanoma cells. Our study could lay a foundation for melanoma treatment through a combined strategy of immunotherapy and chemotherapy.

Graphical abstract

Keywords

Baicalin; Baicalin-loaded PLGA nanoparticles; Dendritic cells activation; Anti-tumor

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