Antibacterial, cytotoxicity and wound healing assessments of amine-functionalised zeolite Y--颗粒学报PARTICUOLOGY

Asraf, M. H., Malek, N. A. N. N., Jemon, K., Sani, N. S., & Muhammad, M. S. (2019). Antibacterial, cytotoxicity and wound healing assessments of amine-functionalised zeolite Y. Particuology, 45, 116-123. https://doi.org/10.1016/j.partic.2018.09.006

Antibacterial, cytotoxicity and wound healing assessments of amine-functionalised zeolite Y

Muhammad Hariz Asraf ^a, Nik Ahmad Nizam Nik Malek ^{a b *}, Khairunadwa Jemon ^a, Nor Suriani Sani ^c, Mustapha Salisu Muhammad ^a

a Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia
b Centre for Sustainable Nanomaterials (CSNano), Ibnu Sina Institute for Scientific and Industrial Research (ISI-ISIR), Universiti Teknologi Malaysia, 81310 UTM Johor, Malaysia
c Office of Deputy Vice Chancellor (Research and Innovation), Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor, Malaysia

10.1016/j.partic.2018.09.006

Volume 45, August 2019, Pages 116-123

Received 13 June 2018, Revised 26 September 2018, Accepted 30 September 2018, Available online 9 March 2019, Version of Record 10 June 2019.

E-mail: niknizam@fbb.utm.my

Highlights

• Zeolite Y was amine-functionalised with (3-aminopropyl) triethoxysilane (APTES).

• APTES-zeolite Y showed antibacterial activity against various ATCC bacteria.

• High amounts of APTES on zeolite Y were cytotoxic against human fibroblast cells.

• High amounts of APTES on zeolite Y reduced cellular wound healing activity.

Abstract

The antibacterial activities, cytotoxicity, and wound healing of amine(3-aminopropyltriethoxysilane (APTES))-functionalised zeolite Y against normal human fibroblast cells were studied. The characterisation of unmodified and amine-functionalised zeolites Y (Z, ZA 0.04, ZA 0.4, and ZA 0.6) by Fourier transform infrared spectroscopy, X-ray powder diffraction, and energy dispersive X-ray analysis proved that the APTES molecules were grafted onto the zeolite Y surface without distorting its framework structure. All amine-functionalised zeolite Y samples possessed antibacterial activities against several ATCC bacteria that were correlated with the increased amount of APTES on the zeolite Y surface. Conversely, when a higher concentration of APTES was grafted on the zeolite Y, higher cytotoxicity was observed against the fibroblast cells. Although the ZA 0.6 sample (zeolite Y functionalised with 0.6 M of APTES) had higher antibacterial activity, it was cytotoxic to the cells. Therefore, the selection of an antibacterial agent for human treatment purposes must also consider its cytotoxicity effect against human cells to ensure it is biocompatible.

Graphical abstract

Keywords

Zeolite Y; Amine functionalised zeolite; Functionalisation; Antibacterial agent; Cytotoxicity

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