A comparison of centrifugation and tangential flow filtration for nanoparticle purification: A case study on acetalated dextran nanoparticles--颗粒学报PARTICUOLOGY

Shah, N. K., Ivone, R., Shen, J., & Meenach, S. A. (2020). A comparison of centrifugation and tangential flow filtration for nanoparticle purification: A case study on acetalated dextran nanoparticles. Particuology, 50, 189-196. https://doi.org/10.1016/j.partic.2019.06.004

A comparison of centrifugation and tangential flow filtration for nanoparticle purification: A case study on acetalated dextran nanoparticles

Nishan K. Shah ^a, Ryan Ivone ^a, Jie Shen ^{a b}, Samantha A. Meenach ^{a b *}

a University of Rhode Island, College of Pharmacy, Department of Biomedical and Pharmaceutical Sciences, Kingston, RI 02881, USA
b University of Rhode Island, College of Engineering, Department of Chemical Engineering, Kingston, RI 02881, USA

10.1016/j.partic.2019.06.004

Volume 50, June 2020, Pages 189-196

Received 3 April 2019, Revised 19 June 2019, Accepted 20 June 2019, Available online 5 August 2019, Version of Record 7 April 2020.

E-mail: smeenach@uri.edu

Highlights

• TFF is a commonly used purification technique in particle engineering.

• There is a lack of understanding of the effect of TFF parameters on NP properties.

• TFF parameters were optimized for NP purification.

• The optimized parameters were used to make NPs containing different model drugs.

• TFF resulted in smaller nanoparticles compared with centrifugation.

Abstract

Tangential flow filtration (TFF) is a purification method commonly used in a multitude of fields, including particle engineering. Currently, there is still a lack of comprehensive understanding of the effects of key TFF parameters on nanoparticle (NP) characteristics (purification outcomes). The present study aimed to investigate the influence of various factors on the characteristics of TFF-purified NP. The most commonly used NP purification method, centrifugation, was studied as a control. A design of experiment approach was implemented to investigate the influence of transmembrane pressure, flow rate, and initial drug loading on NP characteristics using paclitaxel as a model small molecule. Following the determination of optimized TFF parameters, the two purification methods were assessed using other model small molecules (i.e. tacrolimus and resveratrol). The results indicated that the TFF parameters and initial loading of the drug played important roles in the purified NP outcomes. Compared with the centrifugation method, TFF resulted in smaller NP with higher drug loading. TFF was able to circumvent issues associated with conventional centrifugation, while yielding similar results, and can potentially serve as a suitable large-scale purification method for polymeric NP.

Graphical abstract

Keywords

Tangential flow filtration; Nanoparticle purification; Box–Behnken design; Acetalated dextran; Drug delivery

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