Artificial oxygen carriers, from nanometer- to micrometer-sized particles, made of hemoglobin composites substituting for red blood cells--颗粒学报PARTICUOLOGY

Matsuhira, T., & Sakai, H. (2022). Artificial oxygen carriers, from nanometer- to micrometer-sized particles, made of hemoglobin composites substituting for red blood cells. Particuology, 64, 43-55. https://doi.org/10.1016/j.partic.2021.08.010

Artificial oxygen carriers, from nanometer- to micrometer-sized particles, made of hemoglobin composites substituting for red blood cells

Takashi Matsuhira, Hiromi Sakai ^*

Department of Chemistry, Nara Medical University, 840 Shijo-cho, Kashihara 634-8521, Japan

10.1016/j.partic.2021.08.010

Volume 64, May 2022, Pages 43-55

Received 14 May 2021, Revised 17 August 2021, Accepted 27 August 2021, Available online 13 September 2021, Version of Record 17 December 2021.

E-mail: hirosakai@naramed-u.ac.jp

Highlights

• Hb-based oxygen carriers (HBOCs) are studied to substitute for red blood cells.

• HBOCs include chemically-modified, genetically-altered, and encapsulated Hbs.

• Size control of HBOCs is a key to suppress side effects of Hb in blood circulation.

• Hb-vesicles (HbV) have a liposomal structure that resembles red blood cell membrane.

• Detailed preclinical studies of HbV clarified the in vivo safety and efficacy.

Abstract

Blood transfusions are regarded as the most well-known and frequently performed cell transplantations. Although current transfusion systems are sophisticated, they cannot be freed from the inherent difficulties that include infection, short shelf life, and blood type mismatching. Artificial oxygen carriers produced using hemoglobin (Hb) are designated as Hb-based oxygen carriers (HBOCs), which are anticipated for use as biomaterials that have potential to resolve issues of transfusion by a radical paradigm shift. Various HBOCs, nanometer-sized to micrometer-sized bioparticles having an oxygen-carrying function, are developed for use as substitutes for red blood cells (RBCs). This paper presents an overview of the classification of HBOCs with reference to their histories, preparations, structures, functions, and in vitro and in vivo properties. Additionally, we give a more detailed introduction of our academic studies of liposome encapsulated Hb, designated as Hb-vesicles (HbV), which mimic the physiologically important corpuscular structure of RBCs. This review outlines perennial efforts and approaches to mimic RBC functions through chemical, genetic, and encapsulation techniques. It will provide important insights into the eventual realization of an alternative for RBC transfusion.

Graphical abstract

Keywords

Blood substitutes; Cross-linking; Hemoglobin (Hb)-based oxygen carriers (HBOCs)Liposome; Supramolecular assembly; Transfusion alternatives

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