In situ biosynthesized silver nanoparticle-incorporated synthesized zeolite A using Orthosiphon aristatus extract for in vitro antibacterial wound healing--颗粒学报PARTICUOLOGY

Asraf, M. H., Sani, N. S., Williams, C. D., Jemon, K., & Malek, N. A. N. N. (2022). In situ biosynthesized silver nanoparticle-incorporated synthesized zeolite A using Orthosiphon aristatus extract for in vitro antibacterial wound healing. Particuology, 67, 27-34. https://doi.org/10.1016/j.partic.2021.09.007

In situ biosynthesized silver nanoparticle-incorporated synthesized zeolite A using Orthosiphon aristatus extract for in vitro antibacterial wound healing

Muhammad Hariz Asraf ^a, Nor Suriani Sani ^b, Craig Denver Williams ^c, Khairunadwa Jemon ^a, Nik Ahmad Nizam Nik Malek ^{a d *}

a Department of Biosciences, Faculty of Science, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia
b Office of Deputy Vice Chancellor (Research and Innovation), Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor, Malaysia
c University of Wolverhampton, Wulfruna Street, Wolverhampton WV1 1SB, England, UK
d Centre for Sustainable Nanomaterials (CSNano), Ibnu Sina Institute for Scientific and Industrial Research (ISI-SIR), Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia

10.1016/j.partic.2021.09.007

Volume 67, August 2022, Pages 27-34

Received 16 July 2021, Revised 8 September 2021, Accepted 14 September 2021, Available online 30 September 2021, Version of Record 29 November 2021.

E-mail: niknizam@utm.my

Highlights

• Incorporation of silver nanoparticles into synthesized zeolite A framework.

• Orthosiphon aristatus leaves extract as a reducing agent for silver ions.

• Yield of bio-green AgNP incorporated on zeolite A through in situ synthesis method.

• Significant antibacterial activity of bio-green AgNP incorporated on zeolite A.

• No cytotoxicity and no hindrance in fibroblast wound healing.

Abstract

The capability of synthesized zeolite A (SZ) to immobilize Ag ions (Ag-SZ) and Ag nanoparticles (AgNp-SZ) were comparatively studied. A novel approach of in situ biosynthesized AgNP-incorporated synthesized zeolite A (AgNp-SZ) was synthesized at an optimum volume of 0.4 mL of the Orthosiphon aristatus (O. aristatus) leaves plant extract (5%) using an in situ approach. In comparison, Ag-SZ was produced by loading the synthesized zeolite with Ag ions. All synthesized materials were characterized for their morphologies and physicochemical properties. The characterization analyses validate that the biosynthesized AgNP (<100 nm) using O. aristatus leaves extract was incorporated into the zeolite A. The antibacterial testing confirmed that these materials have antibacterial activity against Escherichia coli ATCC 11229 and Staphylococcus aureus ATCC 6538. MIC/MBC analysis demonstrated that in 0.9% saline solution, AgNP-SZ had higher antibacterial activity than Ag-SZ. The in vitro cell viability and migration assays were further examined towards human skin fibroblast cells HSF 1184. Results show that the materials are not cytotoxic to HSF 1184, and the biosynthesized AgNP-SZ promotes cell migration and proliferation higher than Ag-SZ. This research proved that the biocompatible antibacterial wound healing agent of AgNP-SZ can be synthesized using an in situ approach where the reduction process of Ag ions in the zeolite A can be performed using plant extract.

Graphical abstract

Keywords

Zeolite; Orthosiphon aristatus; Biosynthesis; Silver nanoparticles; AntibacterialIn vitro wound healing

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