Facile biogenic selenium nanoparticle synthesis, characterization and effects on oxidative stress generated by UV in microalgae--颗粒学报PARTICUOLOGY

Dinc, S. K., Vural, O. A., Kayhan, F. E., & San Keskin, N. O. (2022). Facile biogenic selenium nanoparticle synthesis, characterization and effects on oxidative stress generated by UV in microalgae. Particuology, 70, 30-42. https://doi.org/10.1016/j.partic.2021.12.005

Facile biogenic selenium nanoparticle synthesis, characterization and effects on oxidative stress generated by UV in microalgae

Sena Kardelen Dinc ^a, Oznur Akbal Vural ^b, Figen Esin Kayhan^c *, Nalan Oya San Keskin ^a *

a Polatlı Science and Literature Faculty, Nanosan Laboratory, Ankara Hacı Bayram Veli University, Ankara 06900, Turkey
b Advanced Technologies Application and Research Center, Hacettepe University, Ankara 06900, Turkey
c Science and Literature Faculty, Biology Department, Marmara University, Istanbul 34722, Turkey

10.1016/j.partic.2021.12.005

Volume 70, November 2022, Pages 30-42

Received 30 November 2021, Revised 25 December 2021, Accepted 29 December 2021, Available online 14 January 2022, Version of Record 25 January 2022.

E-mail: fekayhan@marmara.edu.tr; nalan.san@hbv.edu.tr

Highlights

• Selenium nanoparticles (SeNPs) were synthesized using supernatant of halophilic bacterium Citricoccus sp..

• Effects of SeNP was investigated on oxidative stress induced by UV in Chlorella vulgaris.

• Changes in H₂O₂, MDA, chlorophyl (a + b) content and cell density were monitored.

• Synthesized SeNPs alleviated the adverse effects of UV stress in microalgae.

Abstract

Since nanoparticle synthesis via chemical and physical methods is expensive and includes hazardous chemicals, biosynthesis has emerged as an environmentally friendly, clean and viable alternative. The present study reports the synthesis of selenium nanoparticles (SeNPs) using Citricoccus sp.. For the production of SeNPs, the influence of some parameters (time, pH, temperature and stirring rate) was studied. Optimum synthesis conditions were found as pH 8, 24 h reaction time, 37 °C and 150 rpm. Synthesized particles were spherical and were 104.46 ± 50.82 nm with a zeta potential of –20.43 ± 0.41 mV. Afterward, the effects of the nanoparticles on oxidative stress biomarkers, hydrogen peroxide (H₂O₂), malondialdehyde (MDA), chlorophyll (a + b) and growth rate, subsequent to UV-C irradiation on Chlorella vulgaris were investigated. In culture contains nanoparticle and UV-C exposed, the amount of H₂O₂ and MDA decreased on the 1st, 3rd and 7th days following UV exposure compared to UV-applied group, while optical density and cell density increased, the amount of chlorophyll (a + b) changes were statistically similar. Consequently, it has been shown that the synthesized SeNPs alleviated the adverse effects of UV stress in microalgae.

Graphical abstract

Keywords

Chlorella vulgaris; Green synthesis; Nanoparticles; Oxidative stress

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