Preparation of slow-release regulated Rs-198 bilayer microcapsules and application of its lyophilized bacterial inoculant on Capsicum annuum L. under salt stress--颗粒学报PARTICUOLOGY

Zhu, S., He, Y., Dong, J., Dong, Y., Li, C., Wu, Z., & Lu, Y. (2023). Preparation of slow-release regulated Rs-198 bilayer microcapsules and application of its lyophilized bacterial inoculant on Capsicum annuum L. under salt stress. Particuology, 79, 54-63. https://doi.org/10.1016/j.partic.2022.11.007

Preparation of slow-release regulated Rs-198 bilayer microcapsules and application of its lyophilized bacterial inoculant on Capsicum annuum L. under salt stress

Shuangxi Zhu ^a, Yanhui He ^a, Jiawei Dong ^a, Yujia Dong ^a, Chun Li ^b, Zhansheng Wu ^a *, Yimeng Lu ^a

a Xi'an Key Laboratory of Textile Chemical Engineering Auxiliaries, School of Environmental and Chemical Engineering, Xi'an Polytechnic University, Xi'an, 710048, China
b Department of Chemical Engineering, Tsinghua University, Beijing, 100084, China

10.1016/j.partic.2022.11.007

Volume 79, August 2023, Pages 54-63

Received 9 October 2022, Revised 27 October 2022, Accepted 11 November 2022, Available online 24 November 2022, Version of Record 24 January 2023.

E-mail: wuzhans@xpu.edu.cn

Highlights

Abstract

The survival adaptation of bacteria in saline soil is poor. The bilayer microcapsules were prepared by secondary embedding of monolayer sodium alginate (NaAlg) - bentonite (Bent) - sodium carboxymethylcellulose (CMC) microcapsules wrapped with plant growth promoting rhizobacteria (PGPR) Pseudomonas putida Rs-198 by chitosan solution to promote the synergistic effect of bilayer microencapsulation and PGPR. The characterization of the Rs-198 bilayer microcapsules showed that the amino and carboxyl groups were cross-linked and a thin layer of chitosan was formed on the outside of the microcapsule. The bilayer microcapsule (Ch-d) with a chitosan concentration of 0.8 wt% and pH 6 showed a slow release of bacteria with a maximum release of 6.06 × 109 cfu/g on the 7th day. The viable bacteria of Ch-d increased by 4.42% after 60 days of storage compared with monolayer microcapsules. The 0.9 wt% l-cysteine, 10 wt% glycerinum, 10 wt% trehalose and 12 wt% soluble starch were added as bacterial protective agents during the process of preparing the Ch-d lyophilized bacterial inoculant (Ch-d LBI). Pot experiments showed that Ch-d LBI exhibited better growth promotion of Capsicum annuum L. under salt stress. Therefore, the bilayer microcapsule as slow-release bacterial inoculant is a potential alternative for sustainable agriculture.

Graphical abstract

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