- Volumes 84-95 (2024)
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Volumes 72-83 (2023)
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Volume 83
Pages 1-258 (December 2023)
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Volume 82
Pages 1-204 (November 2023)
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Volume 81
Pages 1-188 (October 2023)
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Volume 80
Pages 1-202 (September 2023)
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Volume 79
Pages 1-172 (August 2023)
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Volume 78
Pages 1-146 (July 2023)
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Volume 77
Pages 1-152 (June 2023)
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Volume 76
Pages 1-176 (May 2023)
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Volume 75
Pages 1-228 (April 2023)
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Volume 74
Pages 1-200 (March 2023)
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Volume 73
Pages 1-138 (February 2023)
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Volume 72
Pages 1-144 (January 2023)
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Volume 83
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Volumes 60-71 (2022)
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Volume 71
Pages 1-108 (December 2022)
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Volume 70
Pages 1-106 (November 2022)
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Volume 69
Pages 1-122 (October 2022)
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Volume 68
Pages 1-124 (September 2022)
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Volume 67
Pages 1-102 (August 2022)
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Volume 66
Pages 1-112 (July 2022)
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Volume 65
Pages 1-138 (June 2022)
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Volume 64
Pages 1-186 (May 2022)
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Volume 63
Pages 1-124 (April 2022)
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Volume 62
Pages 1-104 (March 2022)
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Volume 61
Pages 1-120 (February 2022)
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Volume 60
Pages 1-124 (January 2022)
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Volume 71
- Volumes 54-59 (2021)
- Volumes 48-53 (2020)
- Volumes 42-47 (2019)
- Volumes 36-41 (2018)
- Volumes 30-35 (2017)
- Volumes 24-29 (2016)
- Volumes 18-23 (2015)
- Volumes 12-17 (2014)
- Volume 11 (2013)
- Volume 10 (2012)
- Volume 9 (2011)
- Volume 8 (2010)
- Volume 7 (2009)
- Volume 6 (2008)
- Volume 5 (2007)
- Volume 4 (2006)
- Volume 3 (2005)
- Volume 2 (2004)
- Volume 1 (2003)
• A new dual enzyme hybrid nanoflowers based on GLOX and HRP was successfully prepared.
• Resultant GLOX&HRP-HNFs exhibited a good linear range (1–100 μM) and a LOD of 0.59 μM for glutamate.
• The biosensor is a promising candidate for the detection of glutamate.
The enzyme hybrid nanoflower has gained interests in biosensors due to their simple synthesis and high efficiency. In this study, glutamate oxidase (GLOX) and horseradish peroxidase (HRP) hybrid nanoflowers (GLOX&HRP-HNFs) were successfully prepared for the detection of glutamic acid (Glu). The effects of the synthesis conditions on the activity of GLOX & HRP-HNFs were investigated. Results revealed that the maximum activity of GLOX&HRP-HNFs was under 4 mM phosphate radical, 2.5 mM MnSO4, 0.04 mg/mL GLOX, and 0.16 mg/mL HRP. After immobilization, no significant differences were observed in optimum pH and temperature values of the GLOX and HRP. The GLOX&HRP-HNFs exhibited higher storage stability and resistance to organic solvents than free GLOX and HRP. Additionally, the GLOX&HRP-HNFs maintained 69% of its primary activity after 6 cycles. More important, the GLOX&HRP-HNFs exhibited a good linear range from 1 to 100 μM (R2 = 0.9979) and a low limit of detection (LOD) of 0.59 μM for glutamate. These results suggest that the GLOX&HRP-HNFs is a promising candidate for applications in biosensing for the detection of glutamate.