A dual enzyme-phosphate hybrid nanoflower for glutamate detection--颗粒学报PARTICUOLOGY

Li, P., Jia, J., Geng, Z., Pang, S., Wang, R., Bilal, M., . . . Jia, S. (2023). A dual enzyme-phosphate hybrid nanoflower for glutamate detection. Particuology, 83, 63-70. https://doi.org/10.1016/j.partic.2023.02.008

A dual enzyme-phosphate hybrid nanoflower for glutamate detection

Peikun Li^a, Jiahui Jia ^a, Zixin Geng ^a, Saizhao Pang ^a, Ruirui Wang ^a, Muhammad Bilal ^c, Hongjie Bian ^b *, Jiandong Cui ^a *, Shiru Jia ^a

a State Key Laboratory of Food Nutrition and Safety, Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin, 300457, China
b Research Center for Fermentation Engineering of Hebei, College of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhang, 050000, China
c Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, PL-60695, Poznan, Poland

10.1016/j.partic.2023.02.008

Volume 83, December 2023, Pages 63-70

Received 7 November 2022, Revised 3 January 2023, Accepted 8 February 2023, Available online 25 February 2023, Version of Record 13 March 2023.

E-mail: bianhongj@126.com; cjd007cn@163.com

Highlights

• A new dual enzyme hybrid nanoflowers based on GLOX and HRP was successfully prepared.

• Resultant GLOX&HRP-HNFs exhibited a good linear range (1–100 μM) and a LOD of 0.59 μM for glutamate.

• The biosensor is a promising candidate for the detection of glutamate.

Abstract

The enzyme hybrid nanoflower has gained interests in biosensors due to their simple synthesis and high efficiency. In this study, glutamate oxidase (GLOX) and horseradish peroxidase (HRP) hybrid nanoflowers (GLOX&HRP-HNFs) were successfully prepared for the detection of glutamic acid (Glu). The effects of the synthesis conditions on the activity of GLOX & HRP-HNFs were investigated. Results revealed that the maximum activity of GLOX&HRP-HNFs was under 4 mM phosphate radical, 2.5 mM MnSO₄, 0.04 mg/mL GLOX, and 0.16 mg/mL HRP. After immobilization, no significant differences were observed in optimum pH and temperature values of the GLOX and HRP. The GLOX&HRP-HNFs exhibited higher storage stability and resistance to organic solvents than free GLOX and HRP. Additionally, the GLOX&HRP-HNFs maintained 69% of its primary activity after 6 cycles. More important, the GLOX&HRP-HNFs exhibited a good linear range from 1 to 100 μM (R² = 0.9979) and a low limit of detection (LOD) of 0.59 μM for glutamate. These results suggest that the GLOX&HRP-HNFs is a promising candidate for applications in biosensing for the detection of glutamate.

Graphical abstract

Keywords

Glutamate oxidase; Horseradish peroxidase; Nanoflowers; Glutamate detection

文章导读