Targeted therapeutic effects of oral magnetically driven pectin nanoparticles containing chlorogenic acid on colon cancer--颗粒学报PARTICUOLOGY

Zhu, H., Zhang, L., Kou, F., Zhao, J., Lei, J., & He, J. (2024). Targeted therapeutic effects of oral magnetically driven pectin nanoparticles containing chlorogenic acid on colon cancer. Particuology, 84, 53-59. https://doi.org/10.1016/j.partic.2023.02.021

Targeted therapeutic effects of oral magnetically driven pectin nanoparticles containing chlorogenic acid on colon cancer

Huatai Zhu ^a, Limei Zhang ^a, Fujia Kou ^b, Jingyang Zhao ^a, Jiandu Lei ^{a *}, Jing He ^{a *}

a Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing, 100083, China
b Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China

10.1016/j.partic.2023.02.021

Volume 84, January 2024, Pages 53-59

Received 6 January 2023, Revised 21 February 2023, Accepted 28 February 2023, Available online 20 March 2023, Version of Record 25 March 2023.

E-mail: ljd2012@bjfu.edu.cn; hejing2008@sina.com

Highlights

• Preparation of magnetically driven pectin particles by ultrasonic emulsification.

• Construction of a pectin-based delivery vector for oral colon cancer therapy.

• Fe₃O₄-carrying vector can be guided to cancer sites under the influence of magnetic fields.

Abstract

Oral colonic nano-drug delivery system has attracted growing attention in treating colon cancer for their excellent characteristics. However, the unique and complex structure of the gastrointestinal tract is still an obstacle to the safe delivery of drugs targeting sites in colon tumors. Here, we designed magnetically driven dual-targeted oral colonic nanoparticles loaded with chlorogenic acid using pectin and oleic acid-modified iron oxide (Fe₃O₄@OA). Specific degradation of pectin by pectinase produced by colonic flora and magnetic fields applied to the colon confers specific targeting of nanoparticles to the colon. In order to overcome the challenge of preparing magnetically driven nanoparticles with small and homogeneous particle sizes by a single conventional method, we developed the combined ultrasound-emulsification technique. The average particle size of the prepared nanoparticles was 81.04 ± 1.02 nm, which showed good drug release in the simulated colonic environment. In vitro anticancer studies, the drug-loaded nanoparticles possess an obvious toxicity and apoptosis-inducing ability against cancer cells. Meanwhile, the hemolysis results demonstrated the safety of the nanoplatform (PET/CGA/Fe₃O₄@OA). This work holds broad prospects as a new treatment modality for colon cancer.

Graphical abstract

Keywords

Pectin; Chlorogenic acid; Ultrasound-emulsion; Magnetically driven; Colon cancer; Colon targeted

文章导读