Optimization on crystallization process of the magnetic core lysozyme--颗粒学报PARTICUOLOGY

Hou, L., Qu, C., Su, M., Liang, Z., & Hao, Q. (2024). Optimization on crystallization process of the magnetic core lysozyme. Particuology, 87, 106-113. https://doi.org/10.1016/j.partic.2023.07.016

Optimization on crystallization process of the magnetic core lysozyme

Lixia Hou, Chunxiao Qu, Min Su ^*, Zhihan Liang, Qi Hao

School of Chemical Engineering, Hebei University of Technology, Tianjin, 300130, China

10.1016/j.partic.2023.07.016

Volume 87, April 2024, Pages 106-113

Received 8 May 2023, Revised 17 July 2023, Accepted 18 July 2023, Available online 5 August 2023, Version of Record 29 August 2023.

E-mail: sumin@tju.edu.cn; sumin@hebut.edu.cn

Highlights

• Crystallization process of magnetic core lysozyme was investigated.

• Original size, modification ratio and additional amounts of the magnetic particles used in the crystallization process were discussed.

• Optimum process conditions for batch crystallization of magnetic core lysozyme were obtained.

• Magnetic core lysozyme crystals with tetragonal morphology and uniform size with a high yield were prepared.

Abstract

The continuous growth of the biomedical market highlights the significance of improvement in the efficiency of batch production for protein drug crystals. Herein, we investigated the effect of polymer-modified Fe₃O₄ nanoparticles on lysozyme (Lys) crystallization at different conditions, using functionalized Fe₃O₄ magnetic nanoparticles (Polymer C@Fe₃O₄) as nucleation agents. The study revealed that the surface charge density of Polymer C@Fe₃O₄ was highest at a modification mass ratio of Fe₃O₄ to Polymer C is 8:3. This ratio facilitates the augmentation of electrostatic interaction and the promotion of crystallization. By adding 15% of the PolymerC@Fe₃O₄ prepared from Fe₃O₄ with an average particle size of 150 nm, the magnetic core lysozyme (M-Lys) crystals with an average particle size of 11.08 μm, narrow size distribution, and regular morphology were obtained in a yield of 82.42% within 60 min.

Graphical abstract

Keywords

Protein; Crystallization; Magnetic particle; Lysozyme

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