Glutamate oxidase-integrated biomimetic Fe3O4 hybrids as cascade nanozymes for glutamate detection--颗粒学报PARTICUOLOGY

Li, P., Wang, Y., Luo, X., Geng, Z., Jia, J., Wang, R., . . . Jia, S. (2024). Glutamate oxidase-integrated biomimetic Fe3O4 hybrids as cascade nanozymes for glutamate detection. Particuology, 88, 192-200. https://doi.org/10.1016/j.partic.2023.09.006

Glutamate oxidase-integrated biomimetic Fe₃O₄ hybrids as cascade nanozymes for glutamate detection

Peikun Li ^{a 1}, Yanchao Wang ^{b 1}, Xiuyan Luo ^a, Zixin Geng ^a, Jiahui Jia ^a, Ruirui Wang ^a, Jiandong Cui ^a^*, Hongjie Bian ^c *, Shiru Jia ^a

a State Key Laboratory of Food Nutrition and Safety, Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin, 300457, China
b Sports Department, Hebei University of Science and Technology, Shijiazhang, 050000, China
c Research Center for Fermentation Engineering of Hebei, College of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhang, 050000, China

10.1016/j.partic.2023.09.006

Volume 88, May 2024, Pages 192-200

Received 4 July 2023, Revised 10 August 2023, Accepted 6 September 2023, Available online 25 September 2023, Version of Record 6 November 2023.

E-mail: cjd007cn@163.com; bianhongj@126.com

Highlights

• A new Glutamate oxidase-integrated biomimetic Fe₃O₄ hybrids was prepared.

• Hybrids exhibited a good linear range of 10–500 μM and a LOD of 6.03 μM for glutamate.

• Glutamate biosensor is a promising candidate for applications in biosensing for detection of glutamate.

Abstract

Herein, a new nanozyme/natural enzyme hybrid biosensor was established for ultrasensitive l-glutamic acid (L-Glu) detection. The Fe₃O₄ nanoparticles with peroxidase-like activity and stability was used as a nanozyme and carrier for immobilizing l-Glutamate oxidase (GLOD) through Schiffff base reaction to construct a chem-enzyme cascade detector. The resultant Fe₃O₄-GOLD exhibited a wide linear range (10−500 μM) and a low detection limit of 6.03 μM for L-Glu detection. Furthermore, the Fe₃O₄-GOLD exhibited excellent pH stability, thermal stability, reusability and storage stability. After repeated nine cycles, Fe₃O₄-GOLD still retained 70% of its initial activity. Meanwhile, Fe₃O₄-GOLD maintained 50% of its initial activity after storage for 20 days, while free GLOD only retained 20% of its initial activity. This strategy of integrating biomimetic Fe₃O₄ and natural enzymes for cascade catalysis makes it possible to design an efficient and stable chemo-enzyme composite catalysts, which are promising for applications in biosensing and biomimetic catalysis.

Graphical abstract

Keywords

Fe₃O₄ nanozyme; GLOD; Chemo-enzymes; Glutamate detection

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