- Volumes 84-95 (2024)
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Volumes 72-83 (2023)
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Volume 83
Pages 1-258 (December 2023)
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Volume 82
Pages 1-204 (November 2023)
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Volume 81
Pages 1-188 (October 2023)
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Volume 80
Pages 1-202 (September 2023)
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Volume 79
Pages 1-172 (August 2023)
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Volume 78
Pages 1-146 (July 2023)
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Volume 77
Pages 1-152 (June 2023)
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Volume 76
Pages 1-176 (May 2023)
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Volume 75
Pages 1-228 (April 2023)
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Volume 74
Pages 1-200 (March 2023)
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Volume 73
Pages 1-138 (February 2023)
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Volume 72
Pages 1-144 (January 2023)
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Volume 83
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Volumes 60-71 (2022)
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Volume 71
Pages 1-108 (December 2022)
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Volume 70
Pages 1-106 (November 2022)
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Volume 69
Pages 1-122 (October 2022)
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Volume 68
Pages 1-124 (September 2022)
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Volume 67
Pages 1-102 (August 2022)
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Volume 66
Pages 1-112 (July 2022)
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Volume 65
Pages 1-138 (June 2022)
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Volume 64
Pages 1-186 (May 2022)
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Volume 63
Pages 1-124 (April 2022)
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Volume 62
Pages 1-104 (March 2022)
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Volume 61
Pages 1-120 (February 2022)
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Volume 60
Pages 1-124 (January 2022)
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Volume 71
- Volumes 54-59 (2021)
- Volumes 48-53 (2020)
- Volumes 42-47 (2019)
- Volumes 36-41 (2018)
- Volumes 30-35 (2017)
- Volumes 24-29 (2016)
- Volumes 18-23 (2015)
- Volumes 12-17 (2014)
- Volume 11 (2013)
- Volume 10 (2012)
- Volume 9 (2011)
- Volume 8 (2010)
- Volume 7 (2009)
- Volume 6 (2008)
- Volume 5 (2007)
- Volume 4 (2006)
- Volume 3 (2005)
- Volume 2 (2004)
- Volume 1 (2003)
• A new Glutamate oxidase-integrated biomimetic Fe3O4 hybrids was prepared.
• Hybrids exhibited a good linear range of 10–500 μM and a LOD of 6.03 μM for glutamate.
• Glutamate biosensor is a promising candidate for applications in biosensing for detection of glutamate.
Herein, a new nanozyme/natural enzyme hybrid biosensor was established for ultrasensitive l-glutamic acid (L-Glu) detection. The Fe3O4 nanoparticles with peroxidase-like activity and stability was used as a nanozyme and carrier for immobilizing l-Glutamate oxidase (GLOD) through Schiffff base reaction to construct a chem-enzyme cascade detector. The resultant Fe3O4-GOLD exhibited a wide linear range (10−500 μM) and a low detection limit of 6.03 μM for L-Glu detection. Furthermore, the Fe3O4-GOLD exhibited excellent pH stability, thermal stability, reusability and storage stability. After repeated nine cycles, Fe3O4-GOLD still retained 70% of its initial activity. Meanwhile, Fe3O4-GOLD maintained 50% of its initial activity after storage for 20 days, while free GLOD only retained 20% of its initial activity. This strategy of integrating biomimetic Fe3O4 and natural enzymes for cascade catalysis makes it possible to design an efficient and stable chemo-enzyme composite catalysts, which are promising for applications in biosensing and biomimetic catalysis.