Surfactant-assisted crystallization of lysozyme and its mechanism--颗粒学报PARTICUOLOGY

Song, Y., Ren, R., Su, M., Liang, Z., & Hao, Q. (2024). Surfactant-assisted crystallization of lysozyme and its mechanism. Particuology, 90, 199-208. https://doi.org/10.1016/j.partic.2023.11.025

Surfactant-assisted crystallization of lysozyme and its mechanism

Yujie Song, Rongfei Ren, Min Su*, Zhihan Liang, Qi Hao

School of Chemical Engineering, Hebei University of Technology, Tianjin 300130, China

10.1016/j.partic.2023.11.025

Volume 90, July 2024, Pages 199-208

Received 3 July 2023, Revised 28 November 2023, Accepted 29 November 2023, Available online 9 December 2023, Version of Record 12 January 2024.

E-mail: sumin@hebut.edu.cn; sumin@tju.edu.cn

Highlights

• Surfactant-assisted lysozyme crystallization process was investigated.

• Betaine hydrochloride induces lysozyme nucleation within 1 min, and the crystal size is grown to 37 μm.

• The mechanism of surfactant-promoting lysozyme crystallization was proposed.

Abstract

Surfactants were used to assist in the crystallization of lysozyme. Three types of surfactants with different structures (sodium dodecyl sulfate, cetyltrimethylammonium bromide, or betaine hydrochloride [BH]) were used. The study indicated that three surfactants were all capable of shortening the crystallization induction time of lysozyme, with BH inducing the shortest crystallization induction time (t = 1 min) and the largest crystal size (37 μm). The molecular interactions among each of the three surfactants aforementioned and lysozyme were also investigated by simulations. It was found that hydrogen bonding dominantly acted between the carboxyl structure of the surfactant BH and the lysozyme, which produced the most favorable conformation for the self-assembly of lysozyme (promote lysozyme orderly aggregation and shorten the crystallization induction time). On this basis, the mechanism of surfactant-promoted lysozyme crystallization was proposed.

Graphical abstract

Keywords

Protein; Crystallization; Surfactant; Lysozyme; Molecular interaction; Mechanism

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