Volume 92
Cheng, Y., Wu, Z., Zhang, B., Zhang, J., Shi, J., & Jiang, Z. (2024). Permeable and robust polymer-silica hybrid armor on cell catalyst for sustainable biomanufacturing. Particuology, 92, 106-112. https://doi.org/10.1016/j.partic.2024.05.002
Permeable and robust polymer-silica hybrid armor on cell catalyst for sustainable biomanufacturing
Yiran Cheng a, Zhenhua Wu a, Boyu Zhang a, Jiaxu Zhang a, Jiafu Shi a b *, Zhongyi Jiang a *
a School of Environmental Science and Engineering, Tianjin University, Tianjin, 300072, China
b Key Laboratory for Green Chemical Technology of Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China
10.1016/j.partic.2024.05.002
Volume 92,
September 2024,
Pages 106-112
Received 13 April 2024, Revised 7 May 2024, Accepted 8 May 2024, Available online 16 May 2024, Version of Record 23 May 2024.
E-mail:
shijiafu@tju.edu.cn; zhyjiang@tju.edu.cn
Highlights
• Polymer-silica hybrid armor (PSHA) on cells is constructed by PEI-induced hydrolysis and condensation of TEOS and APTES.
• Cell@PSHA features excellent permeability, exhibiting catalytic activity of 88.24% compared to free cell.
• Cell@PSHA features excellent stability and catalyze continuous conversion of starch to tagatose for 15 batches over 969 h.
Abstract
Inactivated cell catalysis is one of several central techniques in green biomanufacturing realm. However, the instability and leakage of enzymes in inactivated cell severely restrict the practical applications of inactivated cell catalysis. Constructing armor on the surface of inactivated cells affords a feasible and effective strategy to enhance the stability of cells while commonly lowering the permeability. Herein, polymer-silica hybrid armor (PSHA) is directly generated on the surface of enzyme-containing cells. The branched structure of PEI enables higher porosity of cell@PSHA, exhibiting 1.52-fold enhancement in substrate permeability by contrast with cell@silica armor (SA). The electrostatic interactions (NH3+ with O−) and hydrogen bonding (N⋯H or O⋯H) interactions between structural units enables higher stability of cell@PSHA, showing 3.13-fold elevation in Young's modulus compared with cell@SA. As a result, the cell@PSHA can catalyze continuous conversion of starch to tagatose for 15 batches over 969 h, with an average yield of 77.76 g L−1.
Graphical abstract
Keywords
Biomanufacturing; Whole cell catalysis; Polymer-silica hybrid armor; Tagatose production; Starch conversion
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